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Sperm Select®System
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från artiklar som behandlar
Sperm Select® System och hyaluronan,
den aktiva substansen i Sperm Select®.
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oss om du vill ha särtryck av artiklarna.
01.
Sbracia M., Grasso J., Sayme N., Stronk J., Huszar G. 1997
Hyaluronic acid substantially increases the retention of motility
in cryopreserved/thawed human spermatozoa.
Human Reproduction 12 (9): 1949-1954
". . . [Hyaluronic Acid] HA improved
the retention of sperm motility in thawed spermatozoa. Indeed,
the motility values after 30 h were ~100% higher in the HA compared
with the control samples. This effect of HA was also evident
in the stabilization of spermatozoa with already declining motility.
After removal of the HA from the incubation medium, significantly
increased motility. . . was still detectable for at least 4 h."
"The
utilization of HA will probably prove beneficial in assisted reproduction:
in intrauterine insemination and in in-vitro fertilization (IVF),
the extended sperm motility and velocity will enhance the fertilizing
efficiency; in intracytoplasmic sperm injection (ICSI), the improved
motility will facilitate the identification of viable spermatozoa."
02.
Sanfilippo J.S. 1995.
9 Noteworthy Devices: A Critic’s Collection.
OBG Management, September: 66-73
"Sperm Select enables the gynecologist to recover high-quality,
motile sperm from semen in less than an hour"
03.
Zimmerman E.R., Robertson K.R, Kim H., Drobnis E.Z., Nakajima
S.T. 1994.
Semen preparation with the Sperm Select system versus a
washing technique.
Fertility and Sterility 61(2):269-275
"The Sperm Select System method of sperm preparation
provides a highly uniform specimen with improved sperm quality."
"When this procedure was compared with a washing technique,
advantages in quantitative and qualitative parameters may be due
to a selection of a subpopulation of superior sperm. Alternatively,
semen processed by washing may be damaged during centrifugation
or include suboptimal sperm from the original ejaculate. . . Self-migration
into sodium hyaluronate, however, does not require further washing
and may reduce damage to sperm during the separation process."
"Migration into hyaluronate also removes cellular and particulate
contaminants, including immotile sperm, which may also be undesirable."
"One advantage of sperm selection by migration through sodium
hyaluronate may be its high viscosity. . . A consequence of the
viscous substrate is that sperm forces generated by flagellar
motions are increased. Sperm migration from the hyaluronate sample
into the secretions of the female tract may be enhanced for these
processed specimens. . . Mucus penetration in vitro is reduced
for sperm in an aqueous culture medium, perhaps as a consequence
of low viscosity."
"A further technical advantage of the Sperm Select System
over washing may be attributed to its simplicity and decreased
initial cost. The self migration technique requires minimal equipment
and technical training."
04. Karlström P.O., Bergh T., Lundkvist
Ö. 1993.
A prospective randomized trial of artificial insemination versus
intercourse in cycles stimulated with human menopausal gonadotropin
or clomiphene citrate.
Fertility and Sterility 59(3):554-559
". . . In our hands an acceptable pregnancy rate can
be achieved when a 1-mL aliquot of semen prepared with hyaluronic
acid yields a concentration of at least 0.5 X 106 /mL
of progressively motile sperm in a volume of at least 0.5 mL."
05.
Lichtmacher A. 1992.
Building A Fertility Practice With IUI.
OBG Management, October: 61-65
"The problem with . . . (washing and centrifugation)
is that it doesn’t weed out the dead or weak sperm. And there’s
some concern that all that rough handling may damage the sperm
thus lowering your odds of success. Besides, not every office
has a centrifuge or a technician available to run it."
". . . the [Sperm Select] swim-up method is simpler . . .There’s
a lot less handling . . .It’s cheaper . . .you are able to
harvest the healthiest sperm. . . It eliminates the need for extensive
laboratory facilities, and also means you don’t have to keep
large supplies of culture solutions and washing solutions on hand.
. . "
"They (patients) like the fact that their primary care Ob/Gyn
can offer the procedure in a familiar environment, rather than
having to refer them to a fertility center."
06.
Mortimer D. 1991.
Sperm preparation techniques and iatrogenic failures of in-vitro
fertilization.
Human Reproduction 6(2):173-176 (Editorial)
". . . migration procedures, such as . . . Sperm Select
have also been shown to provide human sperm populations suitable
for IVF. . . there was a significant improvement in pregnancy
rates from 9 to 31%."
"There is now incontrovertible evidence that the centrifugal
pelletting of unselected human sperm populations causes irreversible
damage to the spermatozoa which can impair the fertilizing ability
of the motile fraction subsequently prepared by swim-up migration.
In extreme cases, notably in men with already reduced sperm quality,
the damage can be so severe as to prevent fertilization."
07.
Karlström P.O., Bakos O., Bergh T., Lundkvist Ö. 1991.
Intrauterine insemination and comparison between two methods of
sperm preparation.
Human Reproduction 6(3):390-395
"The new method of sperm preparation (self migration
in sodium hyaluronate) gives an equal number of pregnancies but
yields more motile spermatozoa than the conventional centrifugation/swim
up method. However, sodium hyaluronate is more convenient and
less time-consuming than centrifugation / swim up, as there is
no need to centrifuge."
08.
Karlström P.O., Hjelm E., Lundkvist Ö. 1991.
Comparison of the ability of two sperm preparation techniques
to remove microbes.
Human Reproduction 6(3):386-389
"Sodium hyaluronate is a substance with high viscosity,
thereby presenting a barrier that is difficult for microbes to
penetrate, similar to the one found when spermatozoa penetrate
the cervical mucus in vivo. . . Self migration in sodium hyaluronate
recovered more progressive motile spermatozoa than centrifugation/swim-up
and was still as effective as the conventional centrifugation
swim-up in eliminating microbes. The former method is also faster
and more convenient, since the centrifugation steps are not included."
"We conclude that sperm preparation by self migration in
sodium hyaluronate is a simple and safe method to remove microbes
and to recover motile spermatozoa."
09.
Huszar G., Willetts M., Corrals M. 1990.
Hyaluronic acid (Sperm Select) improves retention of sperm motility
and velocity in normospermic and oligospermic specimens.
Fertility and Sterility 54(6):1127-1134
". . . Sperm Select improves the retention of sperm motility
(most prominently velocity) apparently due to a direct action
of hyaluronic acid on sperm metabolism or contractility. . . Sperm
Select may benefit sperm cryopreservation or the recovery of sperm
motility after thawing."
"From the point of increased chances for fertilization, Sperm
Select compared with the control samples in a time-related fashion
improves the motile sperm density, sperm velocity coefficient,
and lateral head displacement coefficient of specimens, which
collectively enhance the frequency of sperm-oocyte encounters
and the thrust for oocyte penetration."
10.
Bower L., Serafini P., Wikland M., Moyer D. 1989.
The Use of Hyaluronic Acid for Sperm Selection - Evaluation of
Sperm Head Structure.
International Journal of Fertility 34(6): 420-424
". . . In the media supplemented with BSA and HA, there
was a significantly reduced content of debris, granulocytes, and
nonviable spermatozoa. ."
". . . Hyaluronic acid is a physiological substance widely
distributed in the matrix of connective tissues. It is found in
high concentrations in the endocervical canal during the ovulatory
period. Consequently, in vitro conditions mimicking the natural
environment would . . . afford a most appropriate milieu."
". . . Supportive evidence for a possible physiological role
of HA on sperm selection has been provided by . . . who observed
an adequate fertilization rate of human oocytes in vitro followed
by a relatively high pregnancy rate (31%)"
11.
Pousette A., Fredrickson B., Gustafson O., Lunell N.O., Nylund
L., Rosenborg L., Slotte H., Akerlöf E. 1988.
Specific progressive motility and degree of acrosomal reaction
of human spermatozoa are increased by hyaluronic acid and decreased
by Percoll.
Abstract from the Nordic Conference on Assisted Reproduction,
Trondheim, Norway
". . .Percoll decreases the motility as well as the acrosomal
reaction of human sperm. Higher concentration of Percoll showed
a more pronounced effect on both variables. As a sharp contrast,
hyaluronic acid increased the motility as well as the number of
sperm undergoing acrosomal reaction. The effect on motility was
seen shortly after the addition of hyaluronic acid. . . . this
should be taken into account when preparing sperm for IVF-ET or
for insemination"
12.
Wikland M., Wik O., Steen Y., Qvist K., Söderlund B., Jansson
P.O. 1987.
A self-migration method for the preparation of sperm for in-vitro
fertilization.
Human Reproduction 2(3):191-195
"Highly purified hyaluronic acid (Sperm Select) resulted
in the recovery of a significantly higher percentage of motile
spermatozoa compared with the traditional centrifugation method
(87.5% vs. 76.1%)."
"When comparing media with and without Hya (hyaluronic acid)
in the self migration method for preparation of normal sperm samples,
the media containing Hya resulted in the recovery of a significantly
higher percentage of motile spermatozoa (89.0% vs. 73.8%)."
13. Sjöblom P., Qvist K., Söderlund B., Wik O., Wikland
M. 1987.
A comparison of the quality of sperm prepared for IVF
with hyaluronic acid and the centrifugation/swim-up procedures
VII ESCO/ESHRE Joint Meeting, Budapest, Hungary (Abstract 74)
". . . In our hands, insemination with spermatozoa, prepared
this way have resulted in more pregnancies than inseminations
performed with spermatozoa prepared by the conventional centrifugation
/ swim-up method."
". . . Since hyaluronic acid has been implicated as an inducer
of capacitation and acrosome reaction, a possible relationship
between those events and swim-up in Sperm Select has been investigated.
.."
14. Ishijima S., Oshio S., Mohri H. 1986.
Flagellar Movement of Human Spermatozoa.
Gamete Research 13: 185-197
". . . hyaluronic acid probably has biochemical as well
as mechanical effects on human sperm motility."
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